Diversity of the 'Candidatus Phytoplasma asteris' and 'Candidatus Phytoplasma fraxini' isolates that infect urban trees in Bogotá, Colombia.
Identifieur interne : 000469 ( Main/Exploration ); précédent : 000468; suivant : 000470Diversity of the 'Candidatus Phytoplasma asteris' and 'Candidatus Phytoplasma fraxini' isolates that infect urban trees in Bogotá, Colombia.
Auteurs : Liliana Franco-Lara [Colombie] ; Jennifer Andrea García [Colombie] ; Yuly Eilen Bernal [Colombie] ; Rubén Adolfo Rodríguez [Colombie]Source :
- International journal of systematic and evolutionary microbiology [ 1466-5034 ] ; 2020.
Abstract
Phytoplasmas have been associated with a disease that affects trees of at least 11 species from different botanic families in Bogotá, Colombia. 'Candidatus Phytoplasma asteris' and 'Candidatus Phytoplasma fraxini' are the major groups of phytoplasma in the area of Bogotá. In this study, the genetic diversity within 'Ca. P. asteris' and 'Ca. P. fraxini' was studied in five urban tree species: Croton species (Euphorbiaceae), Fraxinus uhdei (Oleaceae), Magnolia grandiflora (Magnoliaceae), Populus nigra (Salicaceae) and Quercus humboldtii (Fagaceae). Analyses of the 16S rRNA gene using nested PCR, RFLP and sequencing showed that phytoplasmas of 'Ca. P. asteris' could be assigned to: subgroup 16SrI-B; a new subgroup named 16SrI-AF, with a restriction pattern similar to that of 16SrI-B; and a new subgroup named 16SrI-AG, with a restriction pattern similar to that of 16SrI-K and 16SrI-AH with a restriction pattern similar to that of 16SrI-AC. 'Ca. P. fraxini' isolates belonged to a new subgroup named 16SrVII-G, with a restriction pattern similar to that of 16SrVII-A. To complement the identification of the phytoplasma strains, we amplified nonribosomal genes such as leuS and secA. Unexpectedly, it was observed that in 16 trees in which 16S rRNA gene analysis showed the presence of 'Ca. P. fraxini' only, the leuS or secA primers amplified sequences exclusively affiliated to 'Ca. P. asteris. In those plants, sequences belonging to 'Ca. P. fraxini' leuS or secA genes were not amplified. The present work contributes to the identification of novel strains of both species in Colombia, and supports previous suggestions that phytoplasmas in South America are highly variable.
DOI: 10.1099/ijsem.0.004553
PubMed: 33174835
Affiliations:
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Lara L" first="Liliana" last="Franco-Lara">Liliana Franco-Lara</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca</wicri:regionArea><wicri:noRegion>Cundinamarca</wicri:noRegion></affiliation></author><author><name sortKey="Garcia, Jennifer Andrea" sort="Garcia, Jennifer Andrea" uniqKey="Garcia J" first="Jennifer Andrea" last="García">Jennifer Andrea García</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca</wicri:regionArea><wicri:noRegion>Cundinamarca</wicri:noRegion></affiliation></author><author><name sortKey="Bernal, Yuly Eilen" sort="Bernal, Yuly Eilen" uniqKey="Bernal Y" first="Yuly Eilen" last="Bernal">Yuly Eilen Bernal</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km2 vía Cajicá-Zipaquirá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km2 vía Cajicá-Zipaquirá</wicri:regionArea><wicri:noRegion>Km2 vía Cajicá-Zipaquirá</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Faculty of Basic and Applied Sciences, Universidad 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type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytoplasmas have been associated with a disease that affects trees of at least 11 species from different botanic families in Bogotá, Colombia. '<i>Candidatus</i> Phytoplasma asteris' and '<i>Candidatus</i> Phytoplasma fraxini' are the major groups of phytoplasma in the area of Bogotá. In this study, the genetic diversity within '<i>Ca</i>. P. asteris' and '<i>Ca</i>. P. fraxini' was studied in five urban tree species: <i>Croton</i> species (Euphorbiaceae), <i>Fraxinus uhdei</i> (Oleaceae), <i>Magnolia grandiflora</i> (Magnoliaceae), <i>Populus nigra</i> (Salicaceae) and <i>Quercus humboldtii</i> (Fagaceae). Analyses of the 16S rRNA gene using nested PCR, RFLP and sequencing showed that phytoplasmas of '<i>Ca</i>. P. asteris' could be assigned to: subgroup 16SrI-B; a new subgroup named 16SrI-AF, with a restriction pattern similar to that of 16SrI-B; and a new subgroup named 16SrI-AG, with a restriction pattern similar to that of 16SrI-K and 16SrI-AH with a restriction pattern similar to that of 16SrI-AC. <i>'Ca</i>. P. fraxini' isolates belonged to a new subgroup named 16SrVII-G, with a restriction pattern similar to that of 16SrVII-A. To complement the identification of the phytoplasma strains, we amplified nonribosomal genes such as <i>leuS</i> and <i>secA</i>. Unexpectedly, it was observed that in 16 trees in which 16S rRNA gene analysis showed the presence of '<i>Ca</i>. P. fraxini' only, the <i>leuS</i> or <i>secA</i> primers amplified sequences exclusively affiliated to '<i>Ca</i>. P. asteris. In those plants, sequences belonging to '<i>Ca</i>. P. fraxini' <i>leuS</i> or <i>secA</i> genes were not amplified. The present work contributes to the identification of novel strains of both species in Colombia, and supports previous suggestions that phytoplasmas in South America are highly variable.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33174835</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1466-5034</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>11</Day></PubDate></JournalIssue><Title>International journal of systematic and evolutionary microbiology</Title><ISOAbbreviation>Int J Syst Evol Microbiol</ISOAbbreviation></Journal><ArticleTitle>Diversity of the <i>'Candidatus</i> Phytoplasma asteris' and <i>'Candidatus</i> Phytoplasma fraxini' isolates that infect urban trees in Bogotá, Colombia.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1099/ijsem.0.004553</ELocationID><Abstract><AbstractText>Phytoplasmas have been associated with a disease that affects trees of at least 11 species from different botanic families in Bogotá, Colombia. '<i>Candidatus</i> Phytoplasma asteris' and '<i>Candidatus</i> Phytoplasma fraxini' are the major groups of phytoplasma in the area of Bogotá. In this study, the genetic diversity within '<i>Ca</i>. P. asteris' and '<i>Ca</i>. P. fraxini' was studied in five urban tree species: <i>Croton</i> species (Euphorbiaceae), <i>Fraxinus uhdei</i> (Oleaceae), <i>Magnolia grandiflora</i> (Magnoliaceae), <i>Populus nigra</i> (Salicaceae) and <i>Quercus humboldtii</i> (Fagaceae). Analyses of the 16S rRNA gene using nested PCR, RFLP and sequencing showed that phytoplasmas of '<i>Ca</i>. P. asteris' could be assigned to: subgroup 16SrI-B; a new subgroup named 16SrI-AF, with a restriction pattern similar to that of 16SrI-B; and a new subgroup named 16SrI-AG, with a restriction pattern similar to that of 16SrI-K and 16SrI-AH with a restriction pattern similar to that of 16SrI-AC. <i>'Ca</i>. P. fraxini' isolates belonged to a new subgroup named 16SrVII-G, with a restriction pattern similar to that of 16SrVII-A. To complement the identification of the phytoplasma strains, we amplified nonribosomal genes such as <i>leuS</i> and <i>secA</i>. Unexpectedly, it was observed that in 16 trees in which 16S rRNA gene analysis showed the presence of '<i>Ca</i>. P. fraxini' only, the <i>leuS</i> or <i>secA</i> primers amplified sequences exclusively affiliated to '<i>Ca</i>. P. asteris. In those plants, sequences belonging to '<i>Ca</i>. P. fraxini' <i>leuS</i> or <i>secA</i> genes were not amplified. The present work contributes to the identification of novel strains of both species in Colombia, and supports previous suggestions that phytoplasmas in South America are highly variable.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Franco-Lara</LastName><ForeName>Liliana</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>García</LastName><ForeName>Jennifer Andrea</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bernal</LastName><ForeName>Yuly Eilen</ForeName><Initials>YE</Initials><AffiliationInfo><Affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km2 vía Cajicá-Zipaquirá, Colombia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodríguez</LastName><ForeName>Rubén Adolfo</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 vía Cajicá-Zipaquirá, Cundinamarca, Colombia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Int J Syst Evol Microbiol</MedlineTA><NlmUniqueID>100899600</NlmUniqueID><ISSNLinking>1466-5026</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">16SrI</Keyword><Keyword MajorTopicYN="N">16SrRNA</Keyword><Keyword MajorTopicYN="N">16SrVII</Keyword><Keyword MajorTopicYN="N">SecA</Keyword><Keyword MajorTopicYN="N">genetic variability</Keyword><Keyword MajorTopicYN="N">leuS</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>12</Hour><Minute>11</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33174835</ArticleId><ArticleId IdType="doi">10.1099/ijsem.0.004553</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Colombie</li></country></list><tree><country name="Colombie"><noRegion><name sortKey="Franco Lara, Liliana" sort="Franco Lara, Liliana" uniqKey="Franco Lara L" first="Liliana" last="Franco-Lara">Liliana Franco-Lara</name></noRegion><name sortKey="Bernal, Yuly Eilen" sort="Bernal, Yuly Eilen" uniqKey="Bernal Y" first="Yuly Eilen" last="Bernal">Yuly Eilen Bernal</name><name sortKey="Bernal, Yuly Eilen" sort="Bernal, Yuly Eilen" uniqKey="Bernal Y" first="Yuly Eilen" last="Bernal">Yuly Eilen Bernal</name><name sortKey="Garcia, Jennifer Andrea" sort="Garcia, Jennifer Andrea" uniqKey="Garcia J" first="Jennifer Andrea" last="García">Jennifer Andrea García</name><name sortKey="Rodriguez, Ruben Adolfo" sort="Rodriguez, Ruben Adolfo" uniqKey="Rodriguez R" first="Rubén Adolfo" last="Rodríguez">Rubén Adolfo Rodríguez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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